High resolution X-ray diffraction studies of Z-DNA
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A.S. Intemann und C.C. Intemann , Prien am Chiemsee
DNA -- Structure., X-ray crystallogr
|Series||Wissenschaftliche Forschungsbeiträge Biologie, Biochemie, Chemie ;, Bd. 42|
|LC Classifications||QD415 .W54 Bd. 42, QP624 .W54 Bd. 42|
|The Physical Object|
|Pagination||256 p. :|
|LC Control Number||90187766|
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Description High resolution X-ray diffraction studies of Z-DNA PDF
The crystal structure of a Z-DNA hexamer duplex d(CGCGCG)2determined at ultra high resolution of Å and refined without restraints, displays a high degree of regularity and rigidity in its stereochemistry, in contrast to the more flexible B-DNA by: Conventional high resolution X-Ray diffraction has been developed into a powerful tool for the nondestructive ex-situ investigation of epitaxial layers, of heterostructures and superlattice systems: Th Cited by: Combined neutron and X-ray diffraction studies of DNA in crystals and solutions Crystals of left-handed Z-DNA [d(CGCGCG)] 2 diffract X-rays to beyond 1 Å resolution, feature a small unit cell.
Knowing the three-dimensional structure of biological macromolecules, such as proteins and DNA, is crucial for understanding the functioning of life. Biological crystallography, the main method of structural biology, which is the branch of biology that studies the structure and spatial organization in biological macromolecules, is based on the study of X-ray diffraction by crystals of Cited by: 2.
A high-resolution X-ray diffraction study has shown that the first iron atom is pentacoordinate, although it maintains an octahedral structure, while the second one is. Powder X-ray diffraction is a standard method of identifying crystal structures.
Mean-square atomic displacement values can be experimentally obtained from various measurements, such as thermal expansion, thermal conductivity, extended x-ray absorption fine structure (EXAFS) and the widths of the x-ray or neutron diffraction peaks. High-resolution powder XRD data can be collected using.
This special issue of Journal of Applied Crystallography includes some highlights of the Conference on High-Resolution X-ray Diffraction and Imaging (XTOP).
The 11th Biennial Conference was held in St Petersburg, Russia, by the Ioffe Physical Technical Institute of the Russian Academy of Sciences, the Russian National Committee for Crystallography, the A.
Shubnikov. High-resolution powder diffraction has assisted phase identifications, provided quantitative data on peak broadening (such as arise from particle size effects or stacking disorder), enabled studies of subtle superstructures and a growing number of ab initio High resolution X-ray diffraction studies of Z-DNA book solutions, and permitted Rietveld refinements of a range of inorganic.
"A high-resolution X-ray diffraction technique was used to obtain detailed secondary structure of a DNA molecule. The characteristics of the DNA showed it was a left-handed helix with a general shape that was longer and narrower than the classic Watson-Crick model of DNA.
A high-resolution X-ray diffraction technique was used to obtain detailed secondary structure of a DNA molecule. The characteristics of the DNA showed it was a left-handed helix with a general shape that was longer and narrower than the classic Watson-Crick model of DNA.
Kennard O. () DNA Structure: Current Results from Single Crystal X-Ray Diffraction Studies. In: Guschlbauer W., Saenger W. (eds) DNA—Ligand Interactions. NATO ASI Series (Series A: Life Sciences), vol We therefore applied joint neutron/X-ray refinement to high-resolution neutron and X-ray diffraction data of Z-DNA crystals (dCGCGCG) (Langan et al., ).
Importantly, we were able to obtain the. High Resolution X-Ray Diffraction (HRXRD) Training • HRXRD is used to analyze epitaxial thin films – Can determine composition, strain/relaxation, lattice parameters (in-plane and out-of-plane), thickness, and defect concentration • X-Ray Reflectivity (XRR) is used to analyze thin films, including amorphous and non-textured films.
In this work, we performed an in situ high-resolution powder X-ray diffraction study of the crystallization of UiO in a recently developed continuous-flow microwave reactor that provided excellent performances in terms of space-time yield and atom economy. The effect of the addition of different amounts of water and acetic acid to the.
X-Ray Diffraction Lab 1. Lab Description and Capabilities. X-ray diffraction and reflectivity apparatus capable of characterizing thin films, surface structures, and materials at high humidity and high temperature; Very fast, high dynamic range 2-D detector capabilities enabling kinetic studies.
X-ray diffraction patterns were taken on precession cameras using an Elliott rotating anode tube as anx-ray source.
The crystals have anorthorhombic unit cell with dimensionsa = A, b = A, andc = A. Thespacegroupis P with 1 molecule per asymmetric unit.
ThetRNAthus crys-tallizes in an elongated unit cell that is one molecule. The three-dimensional structure of porcine (p) GH was determined (Fig. 2) using single crystal X-ray diffraction techniques to a resolution of A (Abdel-Meguid et al., ).
It was found to contain four antiparallel α -helical regions. This is the first textbook describing crystal structure determination (especially inorganic) from high-resolution transmission electron microscopy (HRTEM) and electron diffraction (ED).
The theoretical background and practical procedures are explained with hundreds of figures. The fundamental fact that the crystallographic structure factor phase information is present in EM images is explained.
The high-spatial-resolution X-ray beam enables diffraction experiments of micrometer-sized samples at multimegabar pressures, such as XRD data collection at more than GPa 16 Y.
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Akahama, N. Hirao, Y. Ohishi, and A. Singh, “ Equation of state of bcc-Mo by static volume compression to GPa,” J. Appl. Phys.(). X-ray Diffraction, by B.E.
Warren, General Publishing Company,(Classic X-ray physics book) Elements of X-ray Diffraction, 3rd Ed., by B.D. Cullity, Addison-Wesley, (Covers most techniques used in traditional materials characterization) High Resolution X-ray Diffractometry and Topography, by D.
Keith Bowen and Brian. An argument that technology accelerates biological discovery, with case studies ranging from chromosome discovery with early microscopes to how DNA replicates using radioisotope labels.
Engineering has been an essential collaborator in biological research and breakthroughs in biology are often enabled by technological advances. Decoding the double helix structure of DNA, for example. In this study, we demonstrated the high-resolution real-space imaging of DNA molecules having the Z- and B-form conformations by frequency-modulation atomic force microscopy (FM-AFM), that has made a great progress in recent years, in an aqueous solution.
The major and minor grooves of both DNA conformations were clearly visualized. Textbooks on X-Ray Diffraction. There are many textbooks that have been published on X-Ray Diffraction. This list covers what I feel are the seminal volumes for X-Ray Powder Diffraction.
I will be adding to this more volumes covering other XRD techniques, such as single crystal diffraction and high-resolution XRD for thin films. High resolution is thus desirable. Knowing the wavelength and the diffraction angle of a reflection, its resolution d can be easily calculated.
This is just a reformulation of the famous Bragg equation X-ray Diffraction Equipment The Experimental Setup To perform an X-ray diffraction experiment, we need an x-ray source. In most cases a. Synchrotron X-Ray diffraction studies Metal hydroxides can be unstable and the crystal structure decomposes when dried from solution or studied with an electron beam.
15,29,30 To avoid drying of sample, XRD was carried out through a capillary in which the nanoparticle solution was injected and studied with a synchrotron light source to achieve. X-ray Diffraction, by B.E.
Warren, General Publishing Company,(Classic x-ray physics book) Elements of X-ray Diffraction,2nd Ed., by B.D. Cullity, Addison-Wesley, (Covers most techniques used in traditional material characterization) High Resolution X-ray Diffractometry and Topography, by D.
Keith Bowen and Brian K. Tanner. The interplay between structure, magnetism, and superconductivity in single crystal Ba(Fe1−xCox)2As2 (x=) has been studied using high-resolution x-ray diffraction by monitoring charge Bragg reflections in each twin domain separately.
The emergence of the superconducting state is correlated with the suppression of the orthorhombic distortion around TC, exhibiting competition between. We have studied the electronic structure of oxidized and reduced CeO 2 () surfaces using x-ray photoelectron spectroscopy (XPS).
The 50 nm thick CeO 2 () film was grown on a YSZ() substrate using oxygen plasma assisted molecular beam epitaxy (OPA-MBE).
This film has been characterized using in situ (RHEED) reflection high energy electron diffraction and ex situ x-ray diffraction.
Details High resolution X-ray diffraction studies of Z-DNA FB2
Among the three DNA conformations, most of the Z-DNA structures showed the highest resolution: % of the Z-DNA structures diffract greater than Å.
In comparison, only % of B-DNA and 14% of A-DNA structures were solved in this high-resolution range. A speckle-based method for the X-ray crystal diffraction wavefront measurement is implemented, and the slope errors of channel-cut crystals with different surface characteristics are measured.
The method uses a speckle scanning technique generated by a scattering membrane translated using a piezo motor to infer the deflection of X-rays from the crystals. The method provides a high angular. This book is intended to give overviews of the relevant X-ray scattering techniques, particularly about inelastic X-ray scattering, elastic scattering, grazing-incidence small-angle X-ray scattering, small-angle X-ray scattering, and high-resolution X-ray diffraction, and, finally, applications of X-ray spectroscopy to study different.The Interpretation of X-ray Diffraction Data • Our (un)certainty about an x-ray structure is directly related to the quality of the x-ray diffraction data.
• The electron density revealed by the Fourier transform of the diffraction data (actually, the square root of the intensities) has resolution-dependent features. High-resolution x-ray scattering study of platinum thin films on sapphire. A Nefedov, A Abromeit, The structural properties of these films have been studied by high-resolution x-ray scattering techniques.
Information about the total thickness of the film and about the surface and interface roughnesses has been determined by means of x-ray.
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